Hybridomas & Monoclonal Antibody Production

Adaltis  can produce purified  and  not purified  antibody  by using  its  cell lines. 

Antibodies   can  be purified   on request  by: HPLC , FPLC , affinity chromatography  and gel  filtration.

For production service information please contact: info@adaltis.net

MONOCLONAL  ANTIBODIES  PRODUCTION  

Method  :  HYBRIDOMA  TECHNOLOGY 

• Hybridoma  Technology developed by Kohler and Milstein has been widely used for the  production of Monoclonal Antibodies.
• Monoclonal Antibodies (MAbs) are antibodies that arise from  a single clone of cells.
• They are homogenous in a plasma cell tumour (Myeloma).
• B cells can produce antibodies of single specificity.
• Myeloma cells or tumor cells are capable of continuous division forming large number of cells .
• The fusion of two types of cells form cells called hybridomas.
• Hybrid cells will be having the property of B cells antibody production and tumour cells  ability of continuous division.
• Hybrid cell ensures antibody production  continuously  in dividing cells.
• The mixture of cells are cultured under  conditions which permit  growth of only hybridoma cells .
• Each hybridoma cell will produce a single type of antibody against a single epitope.
• The hybridoma cells producing the desired monoclonal antibody are the  cultured
• Monoclonal antibodies are isolated and purified.