BLUE STAR

Product Description

                   
• TMB solution  is a  chromogenic reagent for peroxidase, designed for ELISA techniques Manual or Automatic systems.
                     
• The solution is ready for use and contains: 3, 3’, 5, 5’- tetramethylbenzidine (TMB), hydrogen peroxide (H2O2), stabilizing agents.
                     
• TMB solution  is a  chromogenic reagent for peroxidase, designed for ELISA techniques Manual or Automatic systems.
                     
• The Reaction develops an intense blue colour that can be read directly (at 650 nm), or a deep yellow colour (read at 450 nm)  after adding an acid solution.
                     
• High sensitivity and low background.
                   

General TMB information:

TMB (3,3´,5,5´-Tetramethylbenzidine) is a chromogen that yields a blue colour when oxidized, typically as a result of oxygen radicals produced by the hydrolysis of hydrogen peroxide by HRP.

For kinetic or non-stopped ELISA assays, the TMB chromogen has maximal absorbance at 370 nm and 650 nm. The colour then changes to yellow with the addition of sulphuric or phosphoric acid with maximum absorbance at 450 nm.

Product Specification

• Ready for use
                     
• Stability  : 25 months.
                     
• Storage: +2-8°C protected from light. Do not freeze.
                     
• pH 3.5 – 3.7
                     
• Blank < 0.050 OD

Product Use

The TMB solution is added at the end of ELISA protocol  to each microplate well:

• Suggested volume: 100 µl / well
                     
• Incubate  at room temperature (protected from light).
                     
• Time suggested: 15 -30 minutes.
                     
• Colour readings can be made as follows:
  Blue colour  without  reaction stop   (kinetics studies).
  Yellow colour   after  reaction stop  (ELISA  method).
                     
• Measuring the absorbance immediately is recommended, within 30 minutes.