MOLgen

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“Molgen DNA MBTC Kit” is an assay kit for the detection of Mycobacterium tuberculosiscomplex DNA by Real-Time PCR method.

“Molgen DNA MBTC Kit” is intended for the detection of DNA of the Mycobacterium tuberculosis complex (containing mycobacteria causing tuberculosis in humans: M. tuberculosis, M. bovis, M. bovis BCG, M. microti, M. Africanum) extracted from clinical specimens using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product.

“Molgen DNA MBTC Kit” is designed for use with block cyclers iQ5 iCycler, CFX96 (Bio-Rad, USA), DT96 (DNA-Technology, Russia).

The assay kit may be used in clinical practice for testing clinical materials (blood plasma, biopsy materials, cerebrospinal fluid, urine, bronchopulmonary lavage, sputum, synovial fluid).

The extraction of DNA can be performed using the extraction kit of MOLgen series (manufactured by Adaltis). When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis.

“Molgen DNA MBTC Kit” contains reagents required for 96 tests, including control samples.

Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.

Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence: it binds to one of the DNA strands in the course of reaction and provides additional specificity of the method. A DNA probe consists of a fluorescence dye at the 5’-end and a fluorescence quencher at the 3’-end that significantly reduces the fluorescence intensity. During the polymerase synthesis of the complementary strand, the probe is cleaved from the 5’-end due to the 5’-3’ nuclease activity of Taq DNA polymerase, the quencher and the dye become separated, thus increasing the fluorescence signal due to accumulation of the reaction product. The detected fluorescence intensity depends on initial quantity of pathogens DNA template in the sample.

The use of Internal Control sample (IC) prevents generation of false negative results associated with possible loss of DNA template during specimen preparation. IC indicates if PCR inhibitors occur in the reaction mixture. IC should be added to each sample (including control samples) prior to the DNA extraction procedure. Amplification and detection of IC influence neither sensitivity nor specificity of the target DNA PCR.

Note: Internal Control sample is added to the specimen during NA extraction step and is used throughout the whole process of NA extraction, amplification, detection.